digital droplet PCR (Bio-Rad)

Specifications:

• Counting of nucleic acid molecules encapsulated in discrete, volumetrically defined water-in-oil droplets
• High precision
• Reliable measurement of of small fold differences in target DNA sequences
• Increased signal to noise ratio
• Enrichment for rare targets by reducing the influence of high copy templates
• Simple quantification without a standard curve

Applications:

• Measurement of miRNAs
• Measuremt of low abundance targets
• Rare event targets (polymorphisms)